Visualizing the spatial organization of monocytes, interstitial macrophages, and tissue-specific macrophages in situ
Résumé
Tissue-resident mononuclear phagocytes (MPs) are an abundant cell population whose localization in situ
reflects their identity. To enable assessment of their heterogeneity, we developed the red/green/blue
(RGB)-Mac mouse based upon combinations of Cx3cr1 and Csf1r reporter transgenes, providing a complete
visualization of their spatial organization in situ. 3D-multi-photon imaging for spatial mapping and spectral
cytometry employing the three markers in combination distinguished tissue-associated monocytes,
tissue-specific macrophages, and three subsets of connective-tissue-associated MPs, including CCR2+
monocyte-derived cell, CX3CR1+, and FOLR2+ interstitial subsets, associated with distinct sub-anatomic territories.
These populations were selectively reduced by blockade of CSF1, CSF2, CCR2, and CX3CR1 and
efficiently reconstitute their spatial distribution after transient myelo-ablation, suggesting an autonomous
regulatory environment. Our findings emphasize the organization of the MP compartment at the subanatomic
level under steady-state conditions, thereby providing a holistic understanding of their relative
heterogeneity across different tissues.
Domaines
Sciences du Vivant [q-bio]
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Petit et al. - 2024 - Visualizing the spatial organization of monocytes,.pdf (9.16 Mo)
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