Evaluation of a new extraction protocol for yeast identification by mass spectrometry - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Journal of Microbiological Methods Année : 2016

Evaluation of a new extraction protocol for yeast identification by mass spectrometry

Résumé

In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling.

Domaines

Mycologie
Fichier principal
Vignette du fichier
gouriet2016.pdf (148.41 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-01478536 , version 1 (30-09-2021)

Licence

Identifiants

Citer

Frédérique Gouriet, Farida Ghiab, Carine Couderc, Fadi Bittar, Hervé Tissot Dupont, et al.. Evaluation of a new extraction protocol for yeast identification by mass spectrometry. Journal of Microbiological Methods, 2016, 129, pp.61-65. ⟨10.1016/j.mimet.2016.08.001⟩. ⟨hal-01478536⟩
109 Consultations
226 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More