Enhanced toxic metal accumulation in engineered bacterial cells expressing Arabidopsis thaliana phytochelatin synthase. - Aix-Marseille Université Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2003

Enhanced toxic metal accumulation in engineered bacterial cells expressing Arabidopsis thaliana phytochelatin synthase.

Abstract

Phytochelatins (PCs) are metal-binding cysteine-rich peptides, enzymatically synthesized in plants and yeasts from glutathione in response to heavy metal stress by PC synthase (EC 2.3.2.15). In an attempt to increase the ability of bacterial cells to accumulate heavy metals, the Arabidopsis thaliana gene encoding PC synthase (AtPCS) was expressed in Escherichia coli. A marked accumulation of PCs was observed in vivo together with a decrease in the glutathione cellular content. When bacterial cells expressing AtPCS were placed in the presence of heavy metals such as cadmium or the metalloid arsenic, cellular metal contents were increased 20- and 50-fold, respectively. We discuss the possibility of using genes of the PC biosynthetic pathway to design bacterial strains or higher plants with increased abilities to accumulate toxic metals, and also arsenic, for use in bioremediation and/or phytoremediation processes.
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Dates and versions

hal-01561180 , version 1 (12-07-2017)

Identifiers

  • HAL Id : hal-01561180 , version 1
  • PUBMED : 12514032

Cite

Sandrine Sauge-Merle, Stéphan Cuiné, Patrick Carrier, Catherine Lecomte-Pradines, Doan-Trung Luu, et al.. Enhanced toxic metal accumulation in engineered bacterial cells expressing Arabidopsis thaliana phytochelatin synthase.. Applied and Environmental Microbiology, 2003, pp.490-4. ⟨hal-01561180⟩
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