Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies

Sodalisglossinidius,oneofthethreetsetseflymaternallyinheritedsymbionts,waspreviouslyshowntofavorflyinfectionbytrypanosomes,theparasitescausinghumansleepingsickness.Amongapopulationoffliestakingatrypanosome-infectedbloodmeal,onlyafewindividualswillacquiretheparasite;theotherswillescapeinfectionandbeconsideredasrefractorytotrypanosomeinfection.TheaimoftheworkwastoinvestigatewhetherflyrefractorinesscouldbeassociatedwithspecificSodalisgeneexpression.ThetranscriptomeofS.glossinidiusharboredbyfliesthatwerefedeitherwithanon-infectedbloodmeal(control)orwithatrypanosome-infectedmealbutthatdidnotdevelopinfectionwereanalyzed,usingmicroarraytechnology,andcompared.Theanalysisusingthemicroarrayprocedureyielded17genesthatwerefoundtohaveasignificantdifferentialexpressionbetweenthetwogroups.Interestingly,allthesegeneswereoverexpressedinself-cured(refractory)flies.Furtheranalysisoffunctionalannotationofthesegenesindicatedthatmostassociatedbiologicalprocesstermswererelatedtometabolicandbiosyntheticprocessesaswellastooxido-reductionmechanisms.Theseresultsevidencetheoccurrenceofmolecularcrosstalkbetweenthedifferentpartners,inducedbythepassageofthetrypanosomesthroughthefly’sguteventhoughtheparasiteswereunabletoestablishinthegutandtodevelopapermanentinfection.

Mots clés

Self-cured tsetse flies Control flies Tripartite interactions Sleeping sickness Tsetse-symbiont-trypanosomes

Domaines

Sciences du Vivant [q-bio] Médecine vétérinaire et santé animale Génétique des populations [q-bio.PE] Parasitologie

Fichier principal

fmicb-05-00255.pdf (1.76 Mo)

Origine	Publication financée par une institution

Lionel Spinelli : Connectez-vous pour contacter le contributeur

https://amu.hal.science/hal-01612141

Soumis le : vendredi 12 octobre 2018-10:21:32

Dernière modification le : mercredi 15 novembre 2023-10:52:15

Archivage à long terme le : dimanche 13 janvier 2019-12:51:05

Dates et versions

hal-01612141 , version 1 (12-10-2018)

Licence

Paternité

Identifiants

HAL Id : hal-01612141 , version 1
DOI : 10.3389/fmicb.2014.00255
IRD : fdi:010062077

Citer

Illiassou Hamidou Soumana, Bernadette Tchicaya, Béatrice B. Loriod, Pascal Rihet, Anne Geiger. Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies. Frontiers in Microbiology, 2014, 5, pp.255. ⟨10.3389/fmicb.2014.00255⟩. ⟨hal-01612141⟩

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