Involvement of calpains in growth factor-mediated migration - Aix-Marseille Université Access content directly
Journal Articles International Journal of Biochemistry and Cell Biology Year : 2006

Involvement of calpains in growth factor-mediated migration

Germain Mazères
  • Function : Author
Laetitia Daury
  • Function : Author
  • PersonId : 1174951
Patrick Cottin
  • Function : Author
Jean-Jacques Brustis
  • Function : Author

Abstract

Previous research in our laboratory has already shown the importance of the role played by ubiquitous calpains during myoblast migration. The aim of this study was to investigate calpain expression during myoblast migration and, to enhance this phenomenon via calpain stimulation. Ubiquitous calpains are members of a large family of calcium-dependent cysteine proteases. They play an important role in numerous biological and pathological phenomena, such as signal transduction, apoptosis, cell-cycle regulation, cell spreading, adhesion, invasion, myogenesis, and motility. Myoblast migration is a crucial step in myogenesis, as it is necessary for myoblast alignment and fusion to form myotubes. This study started by examining changes in calpain expression during migration, then investigated the possibility of activating myoblast migration via the stimulation of calpain expression and/or activity. The migration rate of myoblasts overexpressing mu- or milli-calpain was quantified. The results showed that calpain overexpression dramatically inhibited myoblast migration. Growth-factor treatments were then used to enhance myoblast migration. The results showed that treatment with IGF-1, TGF-beta1, or insulin induced a major increase in migration and caused a significant increase in m-calpain expression and activity. The increase in migration was totally inhibited by adding calpeptin, a calpain-specific inhibitor. These findings suggest that milli-calpain is involved in growth factor-mediated migration.

Dates and versions

hal-01740983 , version 1 (22-03-2018)

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Ludovic Leloup, Germain Mazères, Laetitia Daury, Patrick Cottin, Jean-Jacques Brustis. Involvement of calpains in growth factor-mediated migration. International Journal of Biochemistry and Cell Biology, 2006, 38 (12), pp.2049 - 2063. ⟨10.1016/j.biocel.2006.04.012⟩. ⟨hal-01740983⟩
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