Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser - Aix-Marseille Université Access content directly
Journal Articles Physical Review Letters Year : 2015

Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser

Martin Kauffmann
Chantal Abergel
Filipe Maia
  • Function : Author
Jean-Michel Claverie
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  • PersonId : 829986
Max Hantke
  • Function : Author
Olof Jönsson
  • Function : Author
Carl Nettelblad
  • Function : Author
Daniel Deponte
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M Marvin Seibert
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N Duane Loh
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Andrew Martin
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  • PersonId : 1008923
John Bozek
Ken Ferguson
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Sascha Epp
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Robert Hartmann
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Nils Kimmel
  • Function : Author
Filipe R. n. c. Maia
  • Function : Author
M. Marvin Seibert
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N. Duane Loh
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Abstract

We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.

Dates and versions

hal-01768282 , version 1 (17-04-2018)

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Cite

Tomas Ekeberg, Martin Kauffmann, Chantal Abergel, Filipe Maia, Virginie Seltzer, et al.. Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser. Physical Review Letters, 2015, 114 (9), pp.098102. ⟨10.1103/PhysRevLett.114.098102⟩. ⟨hal-01768282⟩
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