Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser
Tomas Ekeberg
(1)
,
Martin Kauffmann
,
Chantal Abergel
(2)
,
Filipe Maia
,
Virginie Seltzer
(3)
,
Jean-Michel Claverie
(2)
,
Max Hantke
,
Olof Jönsson
,
Carl Nettelblad
,
Gijs van Der Schot
(4)
,
Mengning Liang
(5)
,
Daniel Deponte
,
Anton Barty
(6)
,
M Marvin Seibert
,
Bianca Iwan
(7)
,
Inger Andersson
(8)
,
N Duane Loh
,
Andrew Martin
(9)
,
Henry Chapman
(10)
,
Christoph Bostedt
(11)
,
John Bozek
(12)
,
Ken Ferguson
,
Jacek Krzywinski
(13)
,
Sascha Epp
,
Daniel Rolles
(14)
,
Artem Rudenko
(15)
,
Robert Hartmann
,
Nils Kimmel
,
Janos Hajdu
(1)
,
Filipe R. n. c. Maia
,
M. Marvin Seibert
,
N. Duane Loh
1
Department of Cell and Molecular Biology [Uppsala]
2 IGS - Information génomique et structurale
3 UPD7 - Université Paris Diderot - Paris 7
4 Bijvoet Center for Biomolecular Research [Utrecht]
5 LCLS - Linac Coherent Light Source
6 LLNL - Lawrence Livermore National Laboratory
7 QUEST - Centre for Quantum Engineering and Space-time Research
8 Department of Molecular Biology
9 University of Oxford
10 DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany
11 ANL - Argonne National Laboratory [Lemont]
12 SSOLEIL - Synchrotron SOLEIL
13 SLAC - SLAC National Accelerator Laboratory
14 DESY - Deutsches Elektronen-Synchrotron [Hamburg]
15 Kansas State University
2 IGS - Information génomique et structurale
3 UPD7 - Université Paris Diderot - Paris 7
4 Bijvoet Center for Biomolecular Research [Utrecht]
5 LCLS - Linac Coherent Light Source
6 LLNL - Lawrence Livermore National Laboratory
7 QUEST - Centre for Quantum Engineering and Space-time Research
8 Department of Molecular Biology
9 University of Oxford
10 DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany
11 ANL - Argonne National Laboratory [Lemont]
12 SSOLEIL - Synchrotron SOLEIL
13 SLAC - SLAC National Accelerator Laboratory
14 DESY - Deutsches Elektronen-Synchrotron [Hamburg]
15 Kansas State University
Martin Kauffmann
- Function : Author
- PersonId : 15011
- IdHAL : martin-kauffmann
Chantal Abergel
- Function : Author
- PersonId : 178853
- IdHAL : chantal-abergel
- ORCID : 0000-0003-1875-4049
- IdRef : 080525741
Filipe Maia
- Function : Author
Max Hantke
- Function : Author
Olof Jönsson
- Function : Author
Carl Nettelblad
- Function : Author
Daniel Deponte
- Function : Author
M Marvin Seibert
- Function : Author
N Duane Loh
- Function : Author
John Bozek
- Function : Author
- PersonId : 785896
- ORCID : 0000-0001-7486-7238
- IdRef : 233749403
Ken Ferguson
- Function : Author
Sascha Epp
- Function : Author
Robert Hartmann
- Function : Author
Nils Kimmel
- Function : Author
Filipe R. n. c. Maia
- Function : Author
M. Marvin Seibert
- Function : Author
N. Duane Loh
- Function : Author
Abstract
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.