Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser - Aix-Marseille Université Access content directly
Journal Articles Physical Review Letters Year : 2015

Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser

Martin Kauffmann
Chantal Abergel
Filipe Maia
  • Function : Author
Jean-Michel Claverie
  • Function : Author
  • PersonId : 829986
Max Hantke
  • Function : Author
Olof Jönsson
  • Function : Author
Carl Nettelblad
  • Function : Author
Daniel Deponte
  • Function : Author
M Marvin Seibert
  • Function : Author
N Duane Loh
  • Function : Author
Andrew Martin
  • Function : Author
  • PersonId : 1008923
John Bozek
Ken Ferguson
  • Function : Author
Sascha Epp
  • Function : Author
Robert Hartmann
  • Function : Author
Nils Kimmel
  • Function : Author
Filipe R. n. c. Maia
  • Function : Author
M. Marvin Seibert
  • Function : Author
N. Duane Loh
  • Function : Author

Abstract

We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.

Dates and versions

hal-01768282 , version 1 (17-04-2018)

Identifiers

Cite

Tomas Ekeberg, Martin Kauffmann, Chantal Abergel, Filipe Maia, Virginie Seltzer, et al.. Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser. Physical Review Letters, 2015, 114 (9), pp.098102. ⟨10.1103/PhysRevLett.114.098102⟩. ⟨hal-01768282⟩
265 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More