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Journal Articles Scientific Data Year : 2016

Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Martin Kauffmann
M Marvin Seibert
  • Function : Author
Chantal Abergel
Filipe Maia
  • Function : Author
Daniel Deponte
  • Function : Author
Andreas Andreasson
  • Function : Author
Olof Jönsson
  • Function : Author
Daniel Westphal
  • Function : Author
Duško Odić
  • Function : Author
Meng Liang
  • Function : Author
Andrew Martin
  • Function : Author
  • PersonId : 1008923
Lars Gumprecht
  • Function : Author
Holger Fleckenstein
  • Function : Author
Saša Bajt
  • Function : Author
Miriam Barthelmess
  • Function : Author
Nicola Coppola
  • Function : Author
  • PersonId : 881456
Jean-Michel Claverie
  • Function : Author
  • PersonId : 829986
N Duane Loh
  • Function : Author
John Bozek
Michael Bogan
  • Function : Author
Christina Hampton
  • Function : Author
Raymond Sierra
  • Function : Author
Robert Shoeman
  • Function : Author
Lukas Lomb
  • Function : Author
Sascha Epp
  • Function : Author
Robert Hartmann
  • Function : Author
Andreas Hartmann
Nils Kimmel
  • Function : Author
Peter Holl
  • Function : Author
Benedikt Rudek
  • Function : Author
Stephan Kassemeyer
  • Function : Author
Lothar Strüder
  • Function : Author
Carlo Schmidt
  • Function : Author
Faton Krasniqi
  • Function : Author
Günter Hauser
  • Function : Author
Christian Reich
  • Function : Author
Heike Soltau
  • Function : Author
Helmut Hirsemann
  • Function : Author
Heinz Graafsma
  • Function : Author
M. Marvin Seibert
  • Function : Author
Filipe R.N.C. Maia
  • Function : Author
N. Duane Loh
  • Function : Author

Abstract

Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.

Dates and versions

hal-01768352 , version 1 (17-04-2018)

Identifiers

Cite

Tomas Ekeberg, Martin Kauffmann, M Marvin Seibert, Chantal Abergel, Filipe Maia, et al.. Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser. Scientific Data , 2016, 3, pp.160060. ⟨10.1038/sdata.2016.60⟩. ⟨hal-01768352⟩
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