Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Tomas Ekeberg
(1)
,
Martin Kauffmann
,
M Marvin Seibert
,
Chantal Abergel
(2)
,
Filipe Maia
,
Virginie Seltzer
(3)
,
Daniel Deponte
,
Andrew Aquila
(4)
,
Andreas Andreasson
,
Bianca Iwan
(5)
,
Olof Jönsson
,
Daniel Westphal
,
Duško Odić
,
Inger Andersson
(6)
,
Anton Barty
(7)
,
Meng Liang
(8)
,
Andrew Martin
(9)
,
Lars Gumprecht
,
Holger Fleckenstein
,
Saša Bajt
,
Miriam Barthelmess
,
Nicola Coppola
(10)
,
Jean-Michel Claverie
(2)
,
N Duane Loh
,
Christoph Bostedt
(11)
,
John Bozek
(12)
,
Jacek Krzywinski
(13)
,
Marc Messerschmidt
(14)
,
Michael Bogan
,
Christina Hampton
,
Raymond Sierra
,
Matthias Frank
(7)
,
Robert Shoeman
,
Lukas Lomb
,
Lutz Foucar
(15)
,
Sascha Epp
,
Daniel Rolles
(16)
,
Artem Rudenko
(17)
,
Robert Hartmann
,
Andreas Hartmann
(18)
,
Nils Kimmel
,
Peter Holl
,
Georg Weidenspointner
(19)
,
Benedikt Rudek
,
Benjamin Erk
(16)
,
Stephan Kassemeyer
,
Ilme Schlichting
(20)
,
Lothar Strüder
,
Joachim Ullrich
(21)
,
Carlo Schmidt
,
Faton Krasniqi
,
Günter Hauser
,
Christian Reich
,
Heike Soltau
,
Sebastian Schorb
(13)
,
Helmut Hirsemann
,
Cornelia Wunderer
(22)
,
Heinz Graafsma
,
Henry Chapman
(23)
,
Janos Hajdu
(1)
,
M. Marvin Seibert
,
Filipe R.N.C. Maia
,
N. Duane Loh
1
Department of Cell and Molecular Biology [Uppsala]
2 IGS - Information génomique et structurale
3 UPD7 - Université Paris Diderot - Paris 7
4 LCLS - Linac Coherent Light Source
5 QUEST - Centre for Quantum Engineering and Space-time Research
6 Department of Molecular Biology
7 LLNL - Lawrence Livermore National Laboratory
8 LIAMA - Brain imaging
9 University of Oxford
10 Public Medicine Infectious Diseases Section
11 ANL - Argonne National Laboratory [Lemont]
12 SSOLEIL - Synchrotron SOLEIL
13 SLAC - SLAC National Accelerator Laboratory
14 NSF - National Science Foundation [Arlington]
15 Max Planck Institute for Medical Research [Heidelberg]
16 DESY - Deutsches Elektronen-Synchrotron [Hamburg]
17 Kansas State University
18 CHU Pitié-Salpêtrière [AP-HP]
19 CESR - Centre d'étude spatiale des rayonnements
20 Department of Biomolecular Mechanisms
21 MPIK - Max-Planck-Institut für Kernphysik
22 SSL - Space Sciences Laboratory [Berkeley]
23 DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany
2 IGS - Information génomique et structurale
3 UPD7 - Université Paris Diderot - Paris 7
4 LCLS - Linac Coherent Light Source
5 QUEST - Centre for Quantum Engineering and Space-time Research
6 Department of Molecular Biology
7 LLNL - Lawrence Livermore National Laboratory
8 LIAMA - Brain imaging
9 University of Oxford
10 Public Medicine Infectious Diseases Section
11 ANL - Argonne National Laboratory [Lemont]
12 SSOLEIL - Synchrotron SOLEIL
13 SLAC - SLAC National Accelerator Laboratory
14 NSF - National Science Foundation [Arlington]
15 Max Planck Institute for Medical Research [Heidelberg]
16 DESY - Deutsches Elektronen-Synchrotron [Hamburg]
17 Kansas State University
18 CHU Pitié-Salpêtrière [AP-HP]
19 CESR - Centre d'étude spatiale des rayonnements
20 Department of Biomolecular Mechanisms
21 MPIK - Max-Planck-Institut für Kernphysik
22 SSL - Space Sciences Laboratory [Berkeley]
23 DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany
Martin Kauffmann
- Function : Author
- PersonId : 15011
- IdHAL : martin-kauffmann
M Marvin Seibert
- Function : Author
Chantal Abergel
- Function : Author
- PersonId : 178853
- IdHAL : chantal-abergel
- ORCID : 0000-0003-1875-4049
- IdRef : 080525741
Filipe Maia
- Function : Author
Daniel Deponte
- Function : Author
Andreas Andreasson
- Function : Author
Olof Jönsson
- Function : Author
Daniel Westphal
- Function : Author
Duško Odić
- Function : Author
Lars Gumprecht
- Function : Author
Holger Fleckenstein
- Function : Author
Saša Bajt
- Function : Author
Miriam Barthelmess
- Function : Author
N Duane Loh
- Function : Author
John Bozek
- Function : Author
- PersonId : 785896
- ORCID : 0000-0001-7486-7238
- IdRef : 233749403
Michael Bogan
- Function : Author
Christina Hampton
- Function : Author
Raymond Sierra
- Function : Author
Robert Shoeman
- Function : Author
Lukas Lomb
- Function : Author
Sascha Epp
- Function : Author
Robert Hartmann
- Function : Author
Andreas Hartmann
- Function : Author
- PersonId : 760489
- ORCID : 0000-0003-0407-742X
Nils Kimmel
- Function : Author
Peter Holl
- Function : Author
Benedikt Rudek
- Function : Author
Stephan Kassemeyer
- Function : Author
Lothar Strüder
- Function : Author
Carlo Schmidt
- Function : Author
Faton Krasniqi
- Function : Author
Günter Hauser
- Function : Author
Christian Reich
- Function : Author
Heike Soltau
- Function : Author
Helmut Hirsemann
- Function : Author
Heinz Graafsma
- Function : Author
M. Marvin Seibert
- Function : Author
Filipe R.N.C. Maia
- Function : Author
N. Duane Loh
- Function : Author
Abstract
Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.