The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux - Aix-Marseille Université Access content directly
Journal Articles Communications Biology Year : 2020

The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux

Abstract

With the spreading of antibiotic resistance, the translocation of antibiotics through bacterial envelopes is crucial for their antibacterial activity. In Gram-negative bacteria, the interplay between membrane permeability and drug efflux pumps must be investigated as a whole. Here, we quantified the intracellular accumulation of a series of fluoroquinolones in population and in individual cells of Escherichia coli according to the expression of the AcrB efflux transporter. Computational results supported the accumulation levels measured experimentally and highlighted how fluoroquinolones side chains interact with specific residues of the distal pocket of the AcrB tight monomer during recognition and binding steps.
Fichier principal
Vignette du fichier
The challenge of intracellular antibiotic accumulation.pdf (1.64 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03220501 , version 1 (07-05-2021)

Licence

Attribution

Identifiers

Cite

Julia Vergalli, Alessio Atzori, Jelena Pajovic, Estelle Dumont, Giuliano Malloci, et al.. The challenge of intracellular antibiotic accumulation, a function of fluoroquinolone influx versus bacterial efflux. Communications Biology, 2020, 3 (1), ⟨10.1038/s42003-020-0929-x⟩. ⟨hal-03220501⟩
49 View
63 Download

Altmetric

Share

Gmail Facebook X LinkedIn More