This paper present simulation-based results on the impact of transmitter (Tx) position and orientation uncertainty on the accuracy of the visible light positioning (VLP) system based on the received signal strength (RSS). There are several constraining factors for RSS-based algorithms, particularly due to multipath channel characteristics and set-up uncertainties. The impact of Tx uncertainties on positioning error performance is studied, assuming a statistical modelling of the uncertainties. Simulation results show that the Tx uncertainties have a severe impact on the positioning error, which can be leveraged through the usage of more transmitters. Concerning a smaller Tx’s position uncertainty of 5 cm, the average positioning errors are 23.3, 15.1, and 13.2 cm with the standard deviation values of 6.4, 4.1, and 2.7 cm for 4-, 9-, and 16-Tx cases, respectively. While for a smaller Tx’ orientation uncertainty of 5°, the average positioning errors are 31.9, 20.6, and 17 cm with standard deviation values of 9.2, 6.3, and 3.9 cm for 4-, 9-, and 16-Tx cases, respectively.