Transcriptional Response in a Sepsis Mouse Model Reflects Transcriptional Response in Sepsis Patients - Aix-Marseille Université Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2022

Transcriptional Response in a Sepsis Mouse Model Reflects Transcriptional Response in Sepsis Patients

Abstract

Mortality due to sepsis remains unacceptably high, especially for septic shock patients. Murine models have been used to better understand pathophysiology mechanisms. However, the mouse model is still under debate. Herein we investigated the transcriptional response of mice injected with lipopolysaccharide (LPS) and compared it to either human cells stimulated in vitro with LPS or to the blood cells of septic patients. We identified a molecular signature composed of 2331 genes with an FDR median of 0%. This molecular signature is highly enriched in regulated genes in peritoneal macrophages stimulated with LPS. There is significant enrichment in several inflammatory signaling pathways, and in disease terms, such as pneumonia, sepsis, systemic inflammatory response syndrome, severe sepsis, an inflammatory disorder, immune suppression, and septic shock. A significant overlap between the genes upregulated in mouse and human cells stimulated with LPS has been demonstrated. Finally, genes upregulated in mouse cells stimulated with LPS are enriched in genes upregulated in human cells stimulated in vitro and in septic patients, who are at high risk of death. Our results support the hypothesis of common molecular and cellular mechanisms between mouse and human sepsis.
Fichier principal
Vignette du fichier
ijms-23-00821.pdf (3.58 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03528060 , version 1 (17-01-2022)

Licence

Attribution

Identifiers

Cite

Florian Rosier, Nicolas Fernandez Nuñez, Magali Torres, Béatrice B. Loriod, Pascal Rihet, et al.. Transcriptional Response in a Sepsis Mouse Model Reflects Transcriptional Response in Sepsis Patients. International Journal of Molecular Sciences, In press, 23 (2), pp.821. ⟨10.3390/ijms23020821⟩. ⟨hal-03528060⟩

Collections

INSERM UNIV-AMU
46 View
91 Download

Altmetric

Share

Gmail Facebook X LinkedIn More