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Journal Articles Neuroscience Letters Year : 1998

Isolation of putative olfactory receptor sequences from pig nasal epithelium

Abstract

Binding to olfactory receptors is the first step in odorant and pheromonal recognition and discrimination. These receptors constitute one of the most important, although poorly known, families of neuronal receptors. In this study we used degenerated oligonucleotides and a RT-PCR approach to selectively amplify olfactory receptors in the nasal epithelium of the domestic pig Sus scrofa. Several combinations of oligonucleotide were tested and allowed the isolation of eleven different partial sequences belonging to the seven transmembrane olfactory receptor family. These receptors formed a separate family within the seven transmembrane receptor superfamily in pigs. Using the criteria of Ben Arie et al. [Ben-Arie N., Lancet D., Taylor C., Khen M., Walker N., Ledbetter DH., Carrozzo R., Patel K., Sheer D., Lehrah H. and North M., Hum. Mol. Genet., 3 (1994) 229-235], the 11 receptors described here can be classified into three known families and seven subfamilies (one known and six new).

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Neurobiology

Dates and versions

hal-03583588 , version 1 (21-02-2022)

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Valery Matarazzo, Alain Tirard, Marielle Renucci, Anne Belaïch, Jean-Luc Clément. Isolation of putative olfactory receptor sequences from pig nasal epithelium. Neuroscience Letters, 1998, 249 (2-3), pp.87-90. ⟨10.1016/S0304-3940(98)00375-9⟩. ⟨hal-03583588⟩

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