Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides - Aix-Marseille Université
Journal Articles Nature Communications Year : 2022

Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides

Lucelia Cabral
  • Function : Author
Gabriela Persinoti
  • Function : Author
Douglas Paixão
  • Function : Author
Marcele Martins
  • Function : Author
Mariana Morais
  • Function : Author
Mariana Chinaglia
  • Function : Author
Mariane Domingues
  • Function : Author
Mauricio Sforca
  • Function : Author
Renan Pirolla
  • Function : Author
Wesley Generoso
  • Function : Author
Clelton Santos
  • Function : Author
Lucas Maciel
  • Function : Author
Mario Murakami
  • Function : Author

Abstract

The largest living rodent, capybara, can efficiently depolymerize and utilize lignocellulosic biomass through microbial symbiotic mechanisms yet elusive. Herein, we elucidate the microbial community composition, enzymatic systems and metabolic pathways involved in the conversion of dietary fibers into short-chain fatty acids, a main energy source for the host. In this microbiota, the unconventional enzymatic machinery from Fibrobacteres seems to drive cellulose degradation, whereas a diverse set of carbohydrate-active enzymes from Bacteroidetes, organized in polysaccharide utilization loci, are accounted to tackle complex hemicelluloses typically found in gramineous and aquatic plants. Exploring the genetic potential of this community, we discover a glycoside hydrolase family of β-galactosidases (named as GH173), and a carbohydrate-binding module family (named as CBM89) involved in xylan binding that establishes an unprecedented three-dimensional fold among associated modules to carbohydrate-active enzymes. Together, these results demonstrate how the capybara gut microbiota orchestrates the depolymerization and utilization of plant fibers, representing an untapped reservoir of enzymatic mechanisms to overcome the lignocellulose recalcitrance, a central challenge toward a sustainable and bio-based economy.
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hal-03589026 , version 1 (06-09-2023)

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Lucelia Cabral, Gabriela Persinoti, Douglas Paixão, Marcele Martins, Mariana Morais, et al.. Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides. Nature Communications, 2022, 13 (1), pp.629. ⟨10.1038/s41467-022-28310-y⟩. ⟨hal-03589026⟩
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