<i>In vitro</i> Applications of the Terpene Mini‐Path 2.0 - Aix-Marseille Université Access content directly
Journal Articles ChemBioChem Year : 2022

In vitro Applications of the Terpene Mini‐Path 2.0

Julie Couillaud
  • Function : Author
Agnès Amouric
  • Function : Author
Elise Courvoisier‐dezord
  • Function : Author
Létitia Leydet
  • Function : Author
Nicolas Schweitzer
  • Function : Author
Marie‐noëlle Rosso
Hayat Hage
  • Function : Author
Margot Loussouarn‐yvon
  • Function : Author
Renaud Vincentelli
  • Function : Author
Jean‐louis Petit
  • Function : Author
Véronique de Berardinis
Mireille Attolini
  • Function : Author
Marc Maresca
Katia Duquesne
  • Function : Author
Gilles Iacazio


In 2019 four groups reported independently the development of a simplified enzymatic access to the diphosphates (IPP and DMAPP) of isopentenol and dimethylallyl alcohol (IOH and DMAOH). The former are the two universal precursors of all terpenes. We report here on an improved version of what we call the terpene mini-path as well as its use in enzymatic cascades in combination with various transferases. The goal of this study is to demonstrate the in vitro utility of the TMP in, i) synthesizing various natural terpenes, ii) revealing the product selectivity of an unknown terpene synthase, or iii) generating unnatural cyclobutylated terpenes.
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Dates and versions

hal-04029565 , version 1 (15-03-2023)



Julie Couillaud, Agnès Amouric, Elise Courvoisier‐dezord, Létitia Leydet, Nicolas Schweitzer, et al.. In vitro Applications of the Terpene Mini‐Path 2.0. ChemBioChem, 2022, 23 (24), pp.e202200595. ⟨10.1002/cbic.202200595⟩. ⟨hal-04029565⟩
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