Multicenter evaluation of light transmission platelet aggregation reagents: communication from the ISTH SSC Subcommittee on Platelet Physiology - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Journal of Thrombosis and Haemostasis Année : 2023

Multicenter evaluation of light transmission platelet aggregation reagents: communication from the ISTH SSC Subcommittee on Platelet Physiology

Carmen Coxon
  • Fonction : Auteur
Monica Bacci
  • Fonction : Auteur
Johannes Raster
  • Fonction : Auteur
Fabio Pulcinelli
  • Fonction : Auteur
Aine Mccormick
  • Fonction : Auteur
Juan Pablo Frontroth
  • Fonction : Auteur
Nuria Bermejo
  • Fonction : Auteur
Alain Stepanian
  • Fonction : Auteur
Maria Cozzi
  • Fonction : Auteur
Anastasia Sveshnikova
  • Fonction : Auteur
Dorothée Faille
  • Fonction : Auteur
Wendy Hollon
  • Fonction : Auteur
Meera Chitlur
  • Fonction : Auteur
Alessandra Casonato
  • Fonction : Auteur
Dominique Lasne
  • Fonction : Auteur
Mathieu Fiore
  • Fonction : Auteur
Marie-Francoise Hurtaud-Roux
  • Fonction : Auteur
Paolo Gresele
  • Fonction : Auteur
Marie Lordkipanidzé
  • Fonction : Auteur

Résumé

Background: Light transmission aggregation (LTA) is used widely by the clinical and research communities. Although it is a gold standard, there is a lack of interlaboratory harmonization. Objectives: The primary objective was to assess whether sources of activators (mainly adenosine diphosphate [ADP], collagen, arachidonic acid, epinephrine, and thrombin receptor activating peptide6) and ristocetin contribute to poor LTA reproducibility. The secondary objective was to evaluate interindividual variability of results to appreciate the distribution of normal values and consequently better interpret pathologic results.Methods: An international multicenter study involving 28 laboratories in which we compared LTA results obtained with center-specific activators and a comparator that we supplied. Results: We report variability in the potency (P) of activators in comparison with the comparator. Thrombin receptor activating peptide 6 (P, 1.32-2.68), arachidonic acid (P, 0.87-1.43), and epinephrine (P, 0.97-1.34) showed the greatest variability. ADP (P, 1.04-1.20) and ristocetin (P, 0.98-1.07) were the most consistent. The data highlighted clear interindividual variability, notably for ADP and epinephrine. Four profiles of responses were observed with ADP from high-responders, intermediate-responders, and low-responders. A fifth profile corresponding to nonresponders (5% of the individuals) was observed with epinephrine.Conclusion: Based on these data, the establishment and adoption of simple standardization principles should mitigate variability due to activator sources. The observation of huge interindividual variability for certain concentrations of activators should lead to a cautious interpretation before reporting a result as abnormal. Confidence can be taken from the fact that difference between sources is not exacerbated in patients treated with antiplatelet agents.

Dates et versions

hal-04459861 , version 1 (15-02-2024)

Identifiants

Citer

Marie-Christine Alessi, Carmen Coxon, Manal Ibrahim-Kosta, Monica Bacci, Sophie Voisin, et al.. Multicenter evaluation of light transmission platelet aggregation reagents: communication from the ISTH SSC Subcommittee on Platelet Physiology. Journal of Thrombosis and Haemostasis, 2023, 21 (9), pp.2596-2610. ⟨10.1016/j.jtha.2023.05.027⟩. ⟨hal-04459861⟩
24 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More