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Article Dans Une Revue International Journal of Molecular Sciences Année : 2024

Exploring the Influence of Zinc Ions on the Conformational Stability and Activity of Protein Disulfide Isomerase

Ana Iochabel Soares Moretti
Andrei Yu Roman
  • Fonction : Auteur
Tiphany Coralie de Bessa
François Devred
Hervé Kovacic

Résumé

The interplay between metal ion binding and the activity of thiol proteins, particularly within the protein disulfide isomerase family, remains an area of active investigation due to the critical role that these proteins play in many vital processes. This research investigates the interaction between recombinant human PDIA1 and zinc ions, focusing on the subsequent implications for PDIA1's conformational stability and enzymatic activity. Employing isothermal titration calorimetry and differential scanning calorimetry, we systematically compared the zinc binding capabilities of both oxidized and reduced forms of PDIA1 and assessed the structural consequences of this interaction. Our results demonstrate that PDIA1 can bind zinc both in reduced and oxidized states, but with significantly different stoichiometry and more pronounced conformational effects in the reduced form of PDIA1. Furthermore, zinc binding was observed to inhibit the catalytic activity of reduced-PDIA1, likely due to induced alterations in its conformation. These findings unveil a potential regulatory mechanism in PDIA1, wherein metal ion binding under reductive conditions modulates its activity. Our study highlights the potential role of zinc in regulating the catalytic function of PDIA1 through conformational modulation, suggesting a nuanced interplay between metal binding and protein stability in the broader context of cellular redox regulation.
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Dates et versions

hal-04458598 , version 1 (14-02-2024)

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Paternité

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Ana Iochabel Soares Moretti, Viktoria E Baksheeva, Andrei Yu Roman, Tiphany Coralie de Bessa, François Devred, et al.. Exploring the Influence of Zinc Ions on the Conformational Stability and Activity of Protein Disulfide Isomerase. International Journal of Molecular Sciences, 2024, 25, ⟨10.3390/ijms25042095⟩. ⟨hal-04458598⟩
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