Prevotella copri and microbiota members mediate the beneficial effects of a therapeutic food for malnutrition - Aix-Marseille Université
Article Dans Une Revue Nature Microbiology Année : 2024

Prevotella copri and microbiota members mediate the beneficial effects of a therapeutic food for malnutrition

Evan Lee
  • Fonction : Auteur
Cyrus Zhou
Kali Pruss
Suzanne Henrissat
  • Fonction : Auteur
Clara Kao
Hannah Lynn
Daniel Webber
Marie Crane
Juan Castillo
Garret Couture
  • Fonction : Auteur
Ye Chen
Nikita Balcazo
Carlito Lebrilla
Olga Ilkayeva
Christopher Newgard
Subhasish Das
  • Fonction : Auteur
Jeffrey Gordon

Résumé

Microbiota-directed complementary food (MDCF) formulations have been designed to repair the gut communities of malnourished children. A randomized controlled trial demonstrated that one formulation, MDCF-2, improved weight gain in malnourished Bangladeshi children compared to a more calorically dense standard nutritional intervention. Metagenome-assembled genomes from study participants revealed a correlation between ponderal growth and expression of MDCF-2 glycan utilization pathways by Prevotella copri strains. To test this correlation, here we use gnotobiotic mice colonized with defined consortia of age- and ponderal growth-associated gut bacterial strains, with or without P. copri isolates closely matching the metagenome-assembled genomes. Combining gut metagenomics and metatranscriptomics with host single-nucleus RNA sequencing and gut metabolomic analyses, we identify a key role of P. copri in metabolizing MDCF-2 glycans and uncover its interactions with other microbes including Bifidobacterium infantis . P. copri -containing consortia mediated weight gain and modulated energy metabolism within intestinal epithelial cells. Our results reveal structure–function relationships between MDCF-2 and members of the gut microbiota of malnourished children with potential implications for future therapies.
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hal-04579760 , version 1 (06-09-2024)

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Hao-Wei Chang, Evan Lee, Yi Wang, Cyrus Zhou, Kali Pruss, et al.. Prevotella copri and microbiota members mediate the beneficial effects of a therapeutic food for malnutrition. Nature Microbiology, 2024, 9 (4), pp.922-937. ⟨10.1038/s41564-024-01628-7⟩. ⟨hal-04579760⟩
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