Diversity and Genetic Reassortment of Keystone Virus in Mosquito Populations in Florida - Aix-Marseille Université
Article Dans Une Revue American Journal of Tropical Medicine and Hygiene Année : 2023

Diversity and Genetic Reassortment of Keystone Virus in Mosquito Populations in Florida

Maha Elbadry
  • Fonction : Auteur
Caroline Efstathion
  • Fonction : Auteur
Whitney Qualls
  • Fonction : Auteur
Massimiliano Tagliamonte
  • Fonction : Auteur
Md. Mahbubul Alam
  • Fonction : Auteur
Md. Siddiqur Rahman Khan
  • Fonction : Auteur
Sadie Ryan
  • Fonction : Auteur
Rui-De Xue
  • Fonction : Auteur
Lea Bangonan
  • Fonction : Auteur
John Lednicky
  • Fonction : Auteur
J. Glenn Morris
  • Fonction : Auteur

Résumé

ABSTRACT. Keystone orthobunyavirus (KEYV), a member of the genus Orthobunyavirus , was first isolated in 1964 from mosquitoes in Keystone, Florida. Although data on human infections are limited, the virus has been linked to a fever/rash syndrome and, possibly, encephalitis, with early studies suggesting that 20% of persons in the Tampa, Florida, region had antibodies to KEYV. To assess the distribution and diversity of KEYV in other regions of Florida, we collected > 6,000 mosquitoes from 43 sampling sites in St. Johns County between June 2019 and April 2020. Mosquitoes were separated into pools by species and collection date and site. All pools with Aedes spp. (293 pools, 2,171 mosquitoes) were screened with a real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assay that identifies KEYV and other closely related virus species of what was previously designated as the California encephalitis serogroup. In 2020, screening for KEYV was expanded to include 211 pools of Culex mosquitoes from sites where KEYV-positive Aedes spp. had been identified. rRT-PCR–positive samples were inoculated into cell cultures, and five KEYV isolates from Aedes atlanticus pools were isolated and sequenced. Analyses of the KEYV large genome segment sequences revealed two distinct KEYV clades, whereas analyses of the medium and small genome segments uncovered past reassortment events. Our data documented the ongoing seasonal circulation of multiple KEYV clades within Ae. atlanticus mosquito populations along the east coast of Florida, highlighting the need for further studies of the impact of this virus on human health.

Dates et versions

hal-04584283 , version 1 (23-05-2024)

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Citer

Maha Elbadry, Caroline Efstathion, Whitney Qualls, Massimiliano Tagliamonte, Md. Mahbubul Alam, et al.. Diversity and Genetic Reassortment of Keystone Virus in Mosquito Populations in Florida. American Journal of Tropical Medicine and Hygiene, 2023, 108 (6), pp.1256-1263. ⟨10.4269/ajtmh.22-0594⟩. ⟨hal-04584283⟩

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