Poster De Conférence Année : 2025

Metabolic characterization of seven bee pollens: molecular network, metabolite isolation and antioxidant assessment

Résumé

In a changing world, bees are facing increasing stressful conditions, such as ozone and pollutant concentrations, and more events of higher temperatures [1–3]. In a project called BeeMed [4], Vanderplanck et al. (CEFE, Montpellier, France) suggested that bees may develop an auto-medication behaviour by selecting the species that may provide them the most beneficial pollen content, notably antioxidant metabolites. The objective of this study, part of the BeeMed project, was to characterize the metabolic content of bee pollens from five dominant species and two mix of species. A Featured-Based Molecular Network (FBMN) was realized after UHPLC-MS/MS analysis to underscore the occurence of species metabolites. Major metabolites from one-species dominant pollen were purified by Centrifugal Partition and preparative chromatography for further chemical characterization (1D and 2D NMR). Extracts and purified metabolites were assessed for their antioxidant properties using an online-antioxidant ABTS assay. Three main spectral families were detected in all bee pollens namely polyamines (ex: spermidines, spermines), flavonoid glycosides and amino-acids. Specific benzylisoquinoline alkaloids (ex: scoulerin and derivatives) were exclusively detected in one-species dominant pollen. "Isorhamnetin-like" dihexoside, exerting antioxidant properties, and "isorhamnetin-like" hexoside were purified from pollen and structurally confirmed. Myricetin dihexoside and p-coumaroyl dicaffeoyl spermidine isomers demonstrated the best antioxidant properties. The use of three complementary approaches (MS-based FBMN, metabolite purification and anti-oxydant assay) permitted to better describe pollen chemical diversity and pave the way for further experiments to better understand bee resilience facing oxidative stresses.

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Dates et versions

hal-05116110 , version 1 (17-06-2025)

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  • HAL Id : hal-05116110 , version 1

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Apolline Hugues, Emie Le Bonhomme, Elnur Garayev, Charlotte Simmler, Stephane Greff. Metabolic characterization of seven bee pollens: molecular network, metabolite isolation and antioxidant assessment. 73rd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA), jointly with Italian Society of Phytochemistry (SIF), Aug 2025, Naples, Italy. ⟨hal-05116110⟩
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