Accuracy and precision of quantitative 31P-MRS measurements of human skeletal muscle mitochondrial function - Aix-Marseille Université Access content directly
Journal Articles AJP - Endocrinology and Metabolism Year : 2016

Accuracy and precision of quantitative 31P-MRS measurements of human skeletal muscle mitochondrial function

Gwenael Layec
  • Function : Author
Jayson R. Gifford
  • Function : Author
Joel D. Trinity
  • Function : Author
Corey R. Hart
  • Function : Author
Ryan S. Garten
  • Function : Author
Song Y. Park
  • Function : Author
Eun-Kee Jeong
  • Function : Author

Abstract

While theoretically sound, the accuracy and precision of (31)P-magnetic resonance spectroscopy ((31)P-MRS) approaches to quantitatively estimate mitochondrial capacity are not well documented. Therefore, employing four differing models of respiratory control (linear, kinetic and multi-point adenosine diphosphate (ADP), and phosphorylation potential), this study sought to determine the accuracy and precision of (31)P-MRS assessments of peak mitochondrial adenosine-triphosphate (ATP) synthesis rate utilizing directly measured peak respiration (State 3) in permeabilized skeletal muscle fibers. In 23 subjects, of different fitness levels, (31)P-MRS during a 24-s maximal isometric knee-extension and high-resolution respirometry in muscle fibers from the vastus lateralis was performed. Although significantly correlated with State 3 respiration (r=0.72), both the linear (45±13 mM.min(-1)) and phosphorylation potential (47±16mM.min(-1)) models grossly overestimated the calculated in vitro peak ATP synthesis rate (P\textless0.05). Of the ADP models, the kinetic model was well correlated with State 3 respiration (r=0.72; P\textless0.05), but moderately overestimated ATP synthesis rate (P\textless0.05), while the multi-point model, although being somewhat less well correlated with State 3 respiration (r=0.55; P\textless0.05), most accurately reflected peak ATP synthesis rate. Of note, the PCr recovery time constant (τ), a qualitative index of mitochondrial capacity, exhibited the strongest correlation with State 3 respiration (r=0.80; P\textless0.05). Therefore, this study reveals that each of the (31)P-MRS data analyses, including PCr τ, exhibit precision in terms of mitochondrial capacity. As only the multi-point ADP model did not overstimate the peak skeletal muscle mitochondrial ATP synthesis, the multi-point ADP model is the only quantitative approach to exhibit both accuracy and precision.

Dates and versions

hal-01425511 , version 1 (03-01-2017)

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Gwenael Layec, Jayson R. Gifford, Joel D. Trinity, Corey R. Hart, Ryan S. Garten, et al.. Accuracy and precision of quantitative 31P-MRS measurements of human skeletal muscle mitochondrial function. AJP - Endocrinology and Metabolism, 2016, ajpendo.00028.2016. ⟨10.1152/ajpendo.00028.2016⟩. ⟨hal-01425511⟩
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