MS/MS Sequencing of Digitally Encoded Poly(alkoxyamine amide)s - Aix-Marseille Université Access content directly
Journal Articles Macromolecules Year : 2015

MS/MS Sequencing of Digitally Encoded Poly(alkoxyamine amide)s

Laurence Charles
Jean-François Lutz

Abstract

Monodisperse sequence-coded oligo(alkoxyamine amide)s were thoroughly investigated by tandem mass spectrometry to evaluate the robustness of this analytical approach as a reliable sequencing methodology. Studied samples were synthesized by orthogonal iterative chemistry on a solid support, and the 0/1 coding system was based on the mass of two amide synthons that alternate with a nitroxide moiety. The major fragmentation pathway experienced by these co-oligomers proceeded via homolysis of all fragile C-ON bonds between a coding unit and a nitroxide moiety. The relative rate of competing C-ON bond cleavages was observed to be sequence-dependent, offering an additional means to validate the sequences reconstructed from the MS/MS fragments. The same fragmentation rules applied for all studied samples, varying in terms of chain length, charge state, encoded sequence, end-groups, and nitroxide moiety. Ion mobility separation was coupled to MS/MS to sequence some more complex co-oligomers composed of both different nitroxides and coding units.
No file

Dates and versions

hal-01460553 , version 1 (07-02-2017)

Identifiers

Cite

Laurence Charles, Chloé Laure, Jean-François Lutz, Raj Kumar Roy. MS/MS Sequencing of Digitally Encoded Poly(alkoxyamine amide)s. Macromolecules, 2015, 48 (13), pp.4319--4328. ⟨10.1021/acs.macromol.5b01051⟩. ⟨hal-01460553⟩
68 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More