The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression - Aix-Marseille Université Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2002

The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression

Abstract

The 3′ Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes—Crip, Crp2, and Mta1—are located ≈70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3′ Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.
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hal-01592717 , version 1 (06-12-2018)

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Jie Zhou, Nasrin Ashouian, Marc Délépine, Fumihiko Matsuda, Christophe Chevillard, et al.. The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99 (21), pp.13693-13698. ⟨10.1073/pnas.212392399⟩. ⟨hal-01592717⟩
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