Recent advances in high-throughput approaches to dissect enhancer function - Aix-Marseille Université
Article Dans Une Revue F1000Research Année : 2017

Recent advances in high-throughput approaches to dissect enhancer function

Résumé

The regulation of gene transcription in higher eukaryotes is accomplished through the involvement of transcription start site (TSS)-proximal (promoters) and -distal (enhancers) regulatory elements. It is now well acknowledged that enhancer elements play an essential role during development and cell differentiation, while genetic alterations in these elements are a major cause of human disease. Many strategies have been developed to identify and characterize enhancers. Here, we discuss recent advances in high-throughput approaches to assess enhancer activity, from the well-established massively parallel reporter assays to the recent clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based technologies. We highlight how these approaches contribute toward a better understanding of enhancer function, eventually leading to the discovery of new types of regulatory sequences, and how the alteration of enhancers can affect transcriptional regulation.
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hal-01612096 , version 1 (28-05-2024)

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David Santiago-Algarra, Lan T. M. Dao, Lydie Pradel, Alexandre España, Salvatore Spicuglia. Recent advances in high-throughput approaches to dissect enhancer function. F1000Research, 2017, 6, pp.939. ⟨10.12688/f1000research.11581.1⟩. ⟨hal-01612096⟩
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