Rapid MALDI-TOF MS identification of commercial truffles - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2019

Rapid MALDI-TOF MS identification of commercial truffles

Résumé

Truffles are edible mushrooms with similar morphological characteristics, that make it difficult to distinguish between highly prized truffles (such as the Périgord black T. melanosporum) and inexpensive truffles (such as the Asian Black T. indicum). These biological and economic features have led to several misidentifications and/or fraudulent profit in the truffle markets. In this paper, we investigate Matrix-assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) biotyping to identify 34 commercial fresh truffles from Europe and Asia. The MALDI-TOF MS clustering rapidly distinguished seven Tuber species identified by ITS phylogenetic analysis. The tasty T. melanosporum was clearly differentiated from the Chinese and less expensive truffles. These cheaper mushrooms were marketed as T. indicum but corresponded to a mix of three species. In total, the method confirmed misidentifications in 26% of commercial specimens. Several unknown blind-coded truffles were rapidly identified, with scores >= 2, using the Bruker Biotyper algorithm against MS databases. This study demonstrates that MALDI-TOF MS is a reliable, rapid and cheaper new tool compared with molecular methods for the identification of truffle species and could be used to control frauds in the truffle markets. It could also be useful for the certification of truffle-inoculated seedlings and/or diversity in forest ecosystems.
Fichier principal
Vignette du fichier
41598_2019_Article_54214.pdf (1.59 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Licence

Dates et versions

hal-02447200 , version 1 (05-07-2024)

Licence

Identifiants

Citer

Khalid El Karkouri, Carine Couderc, Philippe Decloquement, Annick Abeille, Didier Raoult. Rapid MALDI-TOF MS identification of commercial truffles. Scientific Reports, 2019, 9 (1), ⟨10.1038/s41598-019-54214-x⟩. ⟨hal-02447200⟩

Collections

IRD CNRS UNIV-AMU
41 Consultations
2 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More