CglB adhesins secreted at bacterial focal adhesions mediate gliding motility - Aix-Marseille Université Access content directly
Preprints, Working Papers, ... Year : 2021

CglB adhesins secreted at bacterial focal adhesions mediate gliding motility

Salim Islam
  • Function : Author
Laetitia My
Nicolas Jolivet
  • Function : Author
Akeisha Belgrave
  • Function : Author
Betty Fleuchot
  • Function : Author
  • PersonId : 767784
  • IdRef : 167065947
Gael Brasseur
Laura Faure
  • Function : Author
Gaurav Sharma
  • Function : Author
David Lemon
  • Function : Author
Fares Saïdi
  • Function : Author
Jean-Bernard Fiche
  • Function : Author
Benjamin Bratton
  • Function : Author
Mitchell Singer
  • Function : Author
Anthony Garza
  • Function : Author
Marcelo Nollmann
Joshua Shaevitz
  • Function : Author
Tam Mignot

Abstract

The predatory deltaproteobacterium Myxococcus xanthus uses a helically-trafficked motor at bacterial focal adhesion (bFA) sites to power gliding motility. Using TIRF and force microscopy, we herein identify the integrin αI-domain-like outer-membrane (OM) lipoprotein CglB as an essential substratum-coupling protein of the gliding motility complex. Similar to most known OM lipoproteins, CglB is anchored on the periplasmic side of the OM and thus a mechanism must exist to secrete it to the cell surface in order for it to interact with the underlying substratum. We reveal this process to be mediated by a predicted OM β-barrel structure of the gliding complex. This OM platform was found to regulate the conformational activation and secretion of CglB across the OM. These data suggest that the gliding complex promotes surface exposure of CglB at bFAs, thus explaining the manner by which forces exerted by inner-membrane motors are transduced across the cell envelope to the substratum; they also uncover a novel protein secretion mechanism, highlighting the ubiquitous connection between secretion and bacterial motility.

Dates and versions

hal-03097119 , version 1 (05-01-2021)

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Salim Islam, Laetitia My, Nicolas Jolivet, Akeisha Belgrave, Betty Fleuchot, et al.. CglB adhesins secreted at bacterial focal adhesions mediate gliding motility. 2021. ⟨hal-03097119⟩
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