The clpP multigenic family in Streptomyces lividans: conditional expression of the clpP3 clpP4 operon is controlled by PopR, a novel transcriptional activator - Aix-Marseille Université Access content directly
Journal Articles Molecular Microbiology Year : 2000

The clpP multigenic family in Streptomyces lividans: conditional expression of the clpP3 clpP4 operon is controlled by PopR, a novel transcriptional activator

Julie Viala
  • Function : Author
  • PersonId : 747080
  • IdHAL : julie-viala
Philippe Mazodier
  • Function : Correspondent author
  • PersonId : 1091312

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Abstract

The clpP genes are widespread among living organisms and encode the proteolytic subunit of the Clp ATP-dependent protease. These genes are present in a single copy in most eubacteria. However, five clpP genes were identified in Streptomyces coelicolor. The clpP1 clpP2 operon was studied: mutations affected the growth cycle in various Streptomyces. Here, we report studies of the expression of the clpP3 clpP4 operon in Streptomyces lividans. The clpP3 operon was induced in a clpP1 mutant strain, and the regulation of expression was investigated in detail. The product of the putative regulator gene, downstream from clpP4, was purified. Gel migration shift assays and DNase I footprinting showed that this protein binds to the clpP3 promoter and recognizes a tandem 6 bp palindromic repeat (TCTGCC-3N-GGCAGA). In vivo, this DNA-binding protein, named PopR, acts as an activator of the clpP3 operon. Studies of popR expression indicate that the regulator is probably controlled at the post-transcriptional level.
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Dates and versions

hal-03269528 , version 1 (24-06-2021)

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Julie Viala, Georges Rapoport, Philippe Mazodier. The clpP multigenic family in Streptomyces lividans: conditional expression of the clpP3 clpP4 operon is controlled by PopR, a novel transcriptional activator. Molecular Microbiology, 2000, 38 (3), pp.602-612. ⟨10.1046/j.1365-2958.2000.02155.x⟩. ⟨hal-03269528⟩
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