Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system - Aix-Marseille Université Access content directly
Journal Articles Acta crystallographica Section F : Structural biology communications [2014-...] Year : 2015

Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system

Julien Stathopulos
  • Function : Author
Christian Cambillau
Alain Roussel
Philippe Leone

Abstract

PorM is a membrane protein involved in the assembly of the type IX secretion system (T9SS) from Porphyromonas gingivalis , a major bacterial pathogen responsible for periodontal disease in humans. The periplasmic domain of PorM was overexpressed in Escherichia coli and purified. A fragment of the purified protein was obtained by limited proteolysis. Crystals of this fragment belonged to the tetragonal space group P 4 3 2 1 2. Native and MAD data sets were recorded to 2.85 and 3.1 Å resolution, respectively, using synchrotron radiation.

Dates and versions

hal-03274774 , version 1 (30-06-2021)

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Julien Stathopulos, Christian Cambillau, E. Cascales, Alain Roussel, Philippe Leone. Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system. Acta crystallographica Section F : Structural biology communications [2014-..], 2015, 71 (1), pp.71-74. ⟨10.1107/S2053230X1402559X⟩. ⟨hal-03274774⟩
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