Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report - Aix-Marseille Université Access content directly
Journal Articles Pathogens Year : 2021

Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report

Madjid Morsli
  • Function : Author
Quentin Kerharo
  • Function : Author
Pierre-Hugues Roche
  • Function : Author
Lucas Troude

Abstract

Current routine real-time PCR methods used for the point-of-care diagnosis of infectious meningitis do not allow for one-shot genotyping of the pathogen, as in the case of deadly Haemophilus influenzae meningitis. Real-time PCR diagnosed H. influenzae meningitis in a 22-year-old male patient, during his hospitalisation following a more than six-metre fall. Using an Oxford Nanopore Technologies real-time sequencing run in parallel to real-time PCR, we detected the H. influenzae genome directly from the cerebrospinal fluid sample in six hours. Furthermore, BLAST analysis of the sequence encoding for a partial DUF417 domain-containing protein diagnosed a non-b serotype, non-typeable H.influenzae belonging to lineage H. influenzae 22.1-21. The Oxford Nanopore metagenomic next-generation sequencing approach could be considered for the point-of-care diagnosis of infectious meningitis, by direct identification of pathogenic genomes and their genotypes/serotypes.
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hal-03280490 , version 1 (22-06-2023)

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Madjid Morsli, Quentin Kerharo, Jeremy Delerce, Pierre-Hugues Roche, Lucas Troude, et al.. Haemophilus influenzae Meningitis Direct Diagnosis by Metagenomic Next-Generation Sequencing: A Case Report. Pathogens, 2021, 10 (4), pp.461. ⟨10.3390/pathogens10040461⟩. ⟨hal-03280490⟩
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