PAD4 Immunization Triggers Anti-Citrullinated Peptide Antibodies in Normal Mice: Analysis With Peptide Arrays - Aix-Marseille Université Access content directly
Journal Articles Frontiers in Immunology Year : 2022

PAD4 Immunization Triggers Anti-Citrullinated Peptide Antibodies in Normal Mice: Analysis With Peptide Arrays

Nathalie Lambert
Jean Roudier
Isabelle Auger

Abstract

The critical immunological event in rheumatoid arthritis (RA) is the production of antibodies to citrullinated proteins (ACPAs), ie proteins on which arginines have been transformed into citrullines by peptidyl arginine deiminases (PAD). In C3H mice, immunization with PAD4 triggers the production of ACPAs. Here, we developed a peptide array to analyze the fine specificity of anti-citrullinated peptide antibodies and used it to characterize the ACPA response after hPAD4 immunization in mice expressing different H-2 haplotypes. Sera from C3H, DBA/2, BALB/c and C57BL/6 mice immunized with human PAD4 (hPAD4) or control-matched mice immunized with phosphate buffered saline (PBS) were used to screen peptide arrays containing 169 peptides from collagen, filaggrin, EBNA, proteoglycan, enolase, alpha and beta fibrinogen, histon and vimentin. Human PAD4 immunization induced antibodies directed against numerous citrullinated peptides from fibrinogen, histon 4 and vimentin. Most peptides were recognized under their arginine and citrullinated forms. DBA/2 and BALB/c mice (H-2d) had the lowest anticitrullinated peptide IgG responses. C3H (H-2k) and BL6 mice (H-2b) had the highest anticitrullinated peptide IgG responses. The newly developed peptide array allows us to characterize the ACPA production after hPAD4 immunization in mice on the H-2d, H-2k or H-2b backgrounds. This sensitive tool will be useful for further studies on mice for prevention of ACPA production by PAD tolerization.
Fichier principal
Vignette du fichier
pdf fimmu-13-840035.pdf (3.83 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03625616 , version 1 (31-03-2022)

Licence

Attribution

Identifiers

Cite

Marie Hemon, Nathalie Lambert, Fanny Arnoux, Jean Roudier, Isabelle Auger. PAD4 Immunization Triggers Anti-Citrullinated Peptide Antibodies in Normal Mice: Analysis With Peptide Arrays. Frontiers in Immunology, 2022, 13 (840035), ⟨10.3389/fimmu.2022.840035⟩. ⟨hal-03625616⟩

Collections

INSERM UNIV-AMU
41 View
29 Download

Altmetric

Share

Gmail Facebook X LinkedIn More