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Journal Articles Clinical Microbiology Reviews Year : 2020

State of the Art in the Culture of the Human Microbiota: New Interests and Strategies

Abstract

The last 5 years have seen a turning point in the study of the gut microbiota with a rebirth of culture-dependent approaches to study the gut microbiota. High-throughput methods have been developed to study bacterial diversity with culture conditions aimed at mimicking the gut environment by using rich media such as YCFA (yeast extract, casein hydrolysate, fatty acids) and Gifu anaerobic medium in an anaerobic workstation, as well as media enriched with rumen and blood and coculture, to mimic the symbiosis of the gut microbiota.

Dates and versions

hal-03636734 , version 1 (11-04-2022)

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Maryam Tidjani Alou, Sabrina Naud, Saber Khelaifia, Marion Bonnet, Jean-Christophe Lagier, et al.. State of the Art in the Culture of the Human Microbiota: New Interests and Strategies. Clinical Microbiology Reviews, 2020, 34 (1), ⟨10.1128/CMR.00129-19⟩. ⟨hal-03636734⟩

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CNRS UNIV-AMU
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