Tryptophan Metabolites Regulate Neuropentraxin 1 Expression in Endothelial Cells - Aix-Marseille Université Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2022

Tryptophan Metabolites Regulate Neuropentraxin 1 Expression in Endothelial Cells

Abstract

In patients with chronic kidney disease (CKD) and in animal models of CKD, the transcription factor Aryl Hydrocabon Receptor (AhR) is overactivated. In addition to the canonical AhR targets constituting the AhR signature, numerous other genes are regulated by this factor. We identified neuronal pentraxin 1 (NPTX1) as a new AhR target. Belonging to the inflammatory protein family, NPTX1 seems of prime interest regarding the inflammatory state observed in CKD. Endothelial cells were exposed to tryptophan-derived toxins, indoxyl sulfate (IS) and indole-3-acetic acid (IAA). The adenine mouse model of CKD was used to analyze NPTX1 expression in the burden of uremia. NPTX1 expression was quantified by RT-PCR and western blot. AhR involvement was analyzed using silencing RNA. We found that IS and IAA upregulated NPTX1 expression in an AhR-dependent way. Furthermore, this effect was not restricted to uremic indolic toxins since the dioxin 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and the tryptophan photoproduct 6-formylindolo[3,2-b]carbazole (FICZ) do the same. In CKD mice, NPTX1 expression was increased in the aorta. Therefore, NPTX1 is a new target of AhR and further work is necessary to elucidate its exact role during CKD.
Fichier principal
Vignette du fichier
ijms-23-02369.pdf (1.61 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Licence : CC BY - Attribution

Dates and versions

hal-03685840 , version 1 (01-09-2023)

Licence

Attribution

Identifiers

Cite

Romain Vial, Stéphane Poitevin, Nathalie Mckay, Stéphane Burtey, Claire Cerini. Tryptophan Metabolites Regulate Neuropentraxin 1 Expression in Endothelial Cells. International Journal of Molecular Sciences, 2022, 23 (4), pp.2369. ⟨10.3390/ijms23042369⟩. ⟨hal-03685840⟩
32 View
9 Download

Altmetric

Share

Gmail Facebook X LinkedIn More