Expression of the First Recombinant Anti-Tumoral Snake Venom Kunitz-Type Serine Protease Inhibitor - Aix-Marseille Université Access content directly
Journal Articles Toxins Year : 2022

Expression of the First Recombinant Anti-Tumoral Snake Venom Kunitz-Type Serine Protease Inhibitor

Maram Morjen
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  • PersonId : 945312
Wassim Moslah
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  • PersonId : 1124901
José Luis
Naziha Marrakchi
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  • PersonId : 948669
Jed Jebali
  • Function : Author
  • PersonId : 1124753

Abstract

PIVL is a Kunitz-type serine protease inhibitor that was previously characterized from Tunisian snake venom, Macrovipera lebetina transmediterranea. It reduced glioblastoma cells’ development and significantly blocked angiogenesis in in-vitro and ex-vivo models. PIVL exerted these effects by interfering with αvβ3 integrin. In order to produce a biological active recombinant, the cDNA cloning and expression of PIVL was performed in Escherichia coli (BL21)-DE3 cells using pET-22b (+) vector. The recombinant PIVL protein (rPIVL) was purified by nickel affinity chromatography and has recognized monoclonal anti-His antibody. Functionally, rPIVL exhibited potent anti-tumor cell effects as well as anti-angiogenesis properties. Interestingly, we found that both native PIVL (nPIVL) and rPIVL modulated PI3/AKT and MAPK signaling pathways. In all, our results showed that we have successfully expressed the first active anti-oncogenic snake venom Kunitz-type protease inhibitor that can be a potential therapeutic drug against glioblastoma, in its native or recombinant form.
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hal-03870820 , version 1 (24-11-2022)

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Maram Morjen, Wassim Moslah, Imen Touihri-Baraketi, Najet Srairi-Abid, José Luis, et al.. Expression of the First Recombinant Anti-Tumoral Snake Venom Kunitz-Type Serine Protease Inhibitor. Toxins, In press, 14 (3), pp.170. ⟨10.3390/toxins14030170⟩. ⟨hal-03870820⟩
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