Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Nature Structural and Molecular Biology Année : 2022

Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis

Résumé

Processivity clamps tether DNA polymerases to DNA, allowing their access to the primer-template junction. In addition to DNA replication, DNA polymerases also participate in various genome maintenance activities, including translesion synthesis (TLS). However, owing to the error-prone nature of TLS polymerases, their association with clamps must be tightly regulated. Here we show that fork-associated ssDNA-binding protein (SSB) selectively enriches the bacterial TLS polymerase Pol IV at stalled replication forks. This enrichment enables Pol IV to associate with the processivity clamp and is required for TLS on both the leading and lagging strands. In contrast, clamp-interacting proteins (CLIPs) lacking SSB binding are spatially segregated from the replication fork, minimally interfering with Pol IV-mediated TLS. We propose that stalling-dependent structural changes within clusters of fork-associated SSB establish hierarchical access to the processivity clamp. This mechanism prioritizes a subset of CLIPs with SSB-binding activity and facilitates their exchange at the replication fork.

Dates et versions

hal-04084974 , version 1 (28-04-2023)

Identifiants

Citer

Seungwoo Chang, Elizabeth S. Thrall, Luisa Laureti, Sadie C. Piatt, Vincent Pagès, et al.. Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis. Nature Structural and Molecular Biology, 2022, 29 (9), pp.932+. ⟨10.1038/s41594-022-00827-2⟩. ⟨hal-04084974⟩
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