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Article Dans Une Revue STAR Protocols Année : 2023

Protocol to image and analyze hippocampal network dynamics in non-anesthetized mouse pups

Résumé

Two-photon calcium imaging is a powerful technique that has revolutionized our understanding of how neural circuit dynamics supports different behaviors and cognitive processes. However, performing imaging during development remains challenging. Here, we provide a protocol to image CA1 neurons in mouse pups as well as a pipeline of analysis to analyze and share the data. We describe steps for intracerebroventricular injection, cranial window surgery, two-photon calcium imaging, and analysis of imaging data.

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Neurobiologie
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Dates et versions

hal-04379625 , version 1 (08-01-2024)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Mirindra R Ratsifandrihamanana, Robin F Dard, Julien Denis, Rosa Cossart, Michel A Picardo. Protocol to image and analyze hippocampal network dynamics in non-anesthetized mouse pups. STAR Protocols, 2023, 4 (4), pp.102760. ⟨10.1016/j.xpro.2023.102760⟩. ⟨hal-04379625⟩
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