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Article Dans Une Revue Sensors Année : 2024

Distortion Correction and Denoising of Light Sheet Fluorescence Images

Résumé

Light Sheet Fluorescence Microscopy (LSFM) has emerged as a valuable tool for neurobiologists, enabling the rapid and high-quality volumetric imaging of mice brains. However, inherent artifacts and distortions introduced during the imaging process necessitate careful enhancement of LSFM images for optimal 3D reconstructions. This work aims to correct images slice by slice before reconstructing 3D volumes. Our approach involves a three-step process: firstly, the implementation of a deblurring algorithm using the work of K. Becker; secondly, an automatic contrast enhancement; and thirdly, the development of a convolutional denoising auto-encoder featuring skip connections to effectively address noise introduced by contrast enhancement, particularly excelling in handling mixed Poisson–Gaussian noise. Additionally, we tackle the challenge of axial distortion in LSFM by introducing an approach based on an auto-encoder trained on bead calibration images. The proposed pipeline demonstrates a complete solution, presenting promising results that surpass existing methods in denoising LSFM images. These advancements hold potential to significantly improve the interpretation of biological data.
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Dates et versions

hal-04546787 , version 1 (15-04-2024)

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Paternité

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Adrien Julia, Rabah Iguernaissi, François J Michel, Valéry Matarazzo, Djamal Merad. Distortion Correction and Denoising of Light Sheet Fluorescence Images. Sensors, 2024, 24 (7), pp.2053. ⟨10.3390/s24072053⟩. ⟨hal-04546787⟩
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