Tamanu oil, obtained from the seeds of Calophyllum inophyllum L. (Calophyllaceae), was traditionally used to cure various skin problems and ailments in French Polynesia (Pétard P, 1986). Nuts and especially oil are also used for skin care (Raharivelomanana et al., 2018). It was reported to treat different kinds of skin affections as well as conjunctivitis and used as natural cosmetic ingredient. Aiming to produce high quality tamanu oil from an eco-friendly process, ecological extraction of this oil from nuts had been carried out using different methods such as ultrasound- assisted and microwave-assisted extractions as well Soxhlet extraction using agro-solvents. Bioactivity-Based Molecular Networking was performed in order to compare their eco-extracts chemical composition and cytotoxic properties. Ultrasound assisted extraction seemed to be an innovative and interesting process to extract tamanu oil yielding a rich extract within bioactive compounds being responsible of its cytotoxic activity shown on different cancer cell lines (IC50< 0.1%V/V): HuH7 (liver), CaCo-2 (colon), MDA-MB-231 (breast), MDA-MB-468 (breast), HCT116 (colon), PC3 (prostate), MCF-7 (breast). The obtained results indicated that the tamanu eco-extracts can be used as an interesting source of natural ingredient for potential nutraceutical and cosmetic applications.